Composite

Part:BBa_K4998038:Design

Designed by: Asimenia Ioannidou   Group: iGEM23_thessaloniki   (2023-10-10)


VapC15 gene composite


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 328
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cyanobacteria have endogenous toxin-antitoxin systems. Taking that into consideration we tried to find a system that did not already exist in our strain of cyanobacterium.


Source

The promoter used for this part originates from Anabaena (Nostoc) sp. PCC 7120. The VapC15 gene originates from Synechocystis sp. PCC 6803 and the rrnB T2 Terminator originates from E.coli.

References

[1]Chaurasia, A. K., Parasnis, A., & Apte, S. K. (2008). An integrative expression vector for strain improvement and environmental applications of the nitrogen fixing cyanobacterium, anabaena sp. strain PCC7120. Journal of Microbiological Methods, 73(2), 133–141. https://doi.org/10.1016/j.mimet.2008.01.013

[2] Fei, Q., Gao, E.-B., Liu, B., Wei, Y., & Ning, D. (2018). A toxin-antitoxin system Vapbc15 from synechocystis sp. PCC 6803 shows distinct regulatory features. Genes, 9(4), 173. https://doi.org/10.3390/genes9040173